Postdoctoral Position in Structural Biology (M/W)

28 Feb 2024
Job Information

Organisation/Company

Inserm

Research Field

Biological sciences » Biology
Biological sciences » Biological engineering

Researcher Profile

Recognised Researcher (R2)

Country

France

Application Deadline

31 Mar 2024 - 23:59 (Europe/Paris)

Type of Contract

Temporary

Job Status

Full-time

Hours Per Week

38

Offer Starting Date

1 May 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

Characterizing receptor kinases' structure and signaling.

Mission – We are looking for ahighly motivated post-doc with expertise in structural biology of membrane proteins, molecular biology and biochemistry to unravel the molecular mechanisms of a receptor kinase associated with human health and diseases.

The project involves a close collaboration between the group of Dr Pierre Maisonneuve at the University of Bordeaux (UMR 5248 CNRS, IECB, France), which investigate the structural mechanisms of protein kinases in human signaling by using cryo-EM and X-Ray crystallography (1-3) and the group of Dr. Xavier Guillory at the Université de Rennes (Inserm U1242, Rennes, France), which aims at deciphering and targeting the molecular mechanisms of activation of a receptor kinase (4,5). The selected candidate will be based in Dr. Maisonneuve's lab in Bordeaux, France. The position is fully funded as part of the INSPIRE1 research program funded by the French National Research Agency (ANR).

Main activities –

• Molecular biology (design and conception of plasmids for protein expression).

• Protein expression in procaryotic (bacteria) and eukaryotic (insect and human) cells.

• Purification of membrane proteins and incorporation in nanodiscs.

• Structural characterization of membrane proteins by single particle cryo-EM.

• Functional and biophysical characterization in vitro of enzymatic activities (kinase & RNase), substrate specificity (RT-qPCR), protein-protein interactions (ITC, fluorescent spectroscopy, SPR, BLI and others).

Required abilities –

• Expertise in protein biochemistry (ideally in membrane proteins)

• Single-particle electron microscopy (cryo-EM)

• Protein expression in human cells.

• Use of detergents, lipids or amphipols. Previous experience on nanodiscs represents a plus.

• Ability to work independently and as a team member.

• Good communication & organizational skills

Environment –

The candidate will join an exciting and multidisciplinary scientific environment with on-site access to cutting-edge facilities in cryo-EM, X-Ray crystallography, NMR, Biochemistry, Biophysics, molecular biology and cell biology.  The Université of Bordeaux is a Top French university. It offers a dynamic and lively international campus with an ideal location just 2 hours from Paris, the sea and the mountains. 

Position –

• Application deadline: 31st of March, proposed start date: May - July 2024

• Required experience: 2 - 8 years after PhD.

• Fixed term contract, 18 months, but the candidate is encouraged to apply for additional postdoctoral funding.

• Salary: Upon experience and according to INSERM salary standards.

Please send CV, cover letter and at least two contacts likely to provide recommendation to:

Dr. Xavier Guillory - [email protected]

Dr. Pierre Maisonneuve - [email protected]

1. Maisonneuve, P., Sahmi, M. et al.  Nat Struct Mol Biol (2024). 2. Posternak, G., Tang, X., Maisonneuve, P. et al. Nat Chem Biol 16, 1170–1178 (2020). 3. Lavoie, H., Sahmi, M., Maisonneuve, P. et al. Nature 554, 549–553 (2018). 4. Pelizzari-Raymundo, D., […], Guillory, X., iScience, 26, 106687 (2023). 5. Langlais, T., […], Guillory, X., Biochem. J., 478, 2953-2975 (2020)

 
 

 

 

Requirements

Research Field

Biological sciences

Education Level

PhD or equivalent

Skills/Qualifications

Required abilities –

• Expertise in protein biochemistry (ideally in membrane proteins)

• Single-particle electron microscopy (cryo-EM)

• Protein expression in human cells.

• Use of detergents, lipids or amphipols. Previous experience on nanodiscs represents a plus.

• Ability to work independently and as a team member.

• Good communication & organizational skills

 
 

 

 

Specific Requirements

• Application deadline: 31st of March, proposed start date: May - July 2024

• Required experience: 2 - 8 years after PhD.

Languages

ENGLISH

Level

Good

Research Field

Biological sciences

Years of Research Experience

4 - 10

Additional Information
Work Location(s)

Number of offers available

1

Company/Institute

Université de Bordeaux, UMR 5248 CNRS, IECB, France

Country

France

City

Bordeaux

Postal Code

33607

Street

2, Rue Robert Escarpit

Geofield

Where to apply

E-mail

[email protected]

STATUS: EXPIRED