Post-doctoral fellow (M/F) for single molecule imaging in live cells

24 Mar 2024
Job Information

Organisation/Company

CNRS

Department

Institut des Sciences Moléculaires d'Orsay

Research Field

Physics

Researcher Profile

First Stage Researcher (R1)

Country

France

Application Deadline

13 Apr 2024 - 23:59 (UTC)

Type of Contract

Temporary

Job Status

Full-time

Hours Per Week

35

Offer Starting Date

1 Jun 2024

Is the job funded through the EU Research Framework Programme?

HE / ERC

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

The Nanobio team is developing new concepts to push back the limits of single molecule localization microscopy, in particular for live cell imaging. This position will involve developing a new concept recently patented for the localisation of individual fluorescent molecules. By encoding the position within a specific illumination, alternative original detection can be implemeted in particular to speed up acquisition, but also overcome the obstacles in terms of in-depth observation. In particular, the aim will be to design new optical implementations for setting up a chirped structured excitation (in 2D then 3D) offering uniqcity between the position and frequency of the detected signal, making it possible to use a single detector alone or in a matrix to analyse a wide field of observation. The aims of this project are to make it possible to observe/trace single molecules at previously unattainable acquisition rates, and also to open up new areas of observation in spectroscopy, by combining position information on a nanometric scale with detection of the spectrum or lifetime of fluorescence. These new developments are based on an initial patented proof of concept, and will be implemented as part of the TimeNanoLive ERC.
The post may be extended for a further 12 months.

The person will contribute to various aspects :
- setting up the optical implementations of this new concept
- testing and validation of the new experimental set-up
- pilot/simulation programming
- characterisation of various samples
- writing articles
- taking part in conferences

This work will be carried out within ISMO (Unité Mixte CNRS / Université Paris-Saclay) as part of a European proejct ERC TimeNanolive ). The laboratory has an on-site culture room and various microscopes for single molecule localization microscopy.

Requirements

Research Field

Physics

Education Level

PhD or equivalent

Languages

FRENCH

Level

Basic

Research Field

Physics

Years of Research Experience

None

Additional Information
Eligibility criteria

- optics
- microscopy
- knowledge of single-molecule imaging (acquisition and processing)
- knowledge of photophysics
- knowledge of cell culture
- python programming

Website for additional job details

https://emploi.cnrs.fr/Offres/CDD/UMR8214-VERSAR-010/Default.aspx

Work Location(s)

Number of offers available

1

Company/Institute

Institut des Sciences Moléculaires d'Orsay

Country

France

City

ORSAY

Geofield

Where to apply

Website

https://emploi.cnrs.fr/Candidat/Offre/UMR8214-VERSAR-010/Candidater.aspx

Contact

City

ORSAY

Website

http://www.ismo.u-psud.fr

STATUS: EXPIRED