M1/M2 internship: Developing Genotype-Conditioned Artificial Drosophila Larvae Behavioural Data Through Bayesian Program Synthesis
Drosophila larvae have emerged as an ideal platform for simultaneously probing behaviour and the underlying neuronal computation1–5 . Modern genetic tools allow efficient activation or silencing of individual and small groups of neurons. Combining these techniques with standardised stimuli over thousands of individuals makes it possible to relate neurons to behaviour causally However, extracting these relationships from massive and noisy recordings requires the development of new statistically robust approaches.
Recently, in experimental settings to probe defensive actions6 or understand the implementation of neuromodulation in small neural neworks7 , larva populations exhibited significant deviations from usual behavioural features. These deviations lead to either the redefinition of features usually associated with these behaviours or to challenges in detecting them.
We aim to develop a Bayesian Program Synthesis8 (BPS) methodology for producing synthetic data that mirrors key characteristics found in experimental recordings. This method will entail an inference phase to identify large-scale parameters characteristic of larva populations. It will incorporate various generative models to either replicate the behavioural traits associated with particular genotypes or create bespoke behavioural features. Additionally, we plan to use a collection of small generative programs to create larva data under specific action conditions. The efficacy of this approach will be demonstrated by applying our behaviour analysis pipeline to the synthetic data and assessing its ability to facilitate transfer learning with annotated behavioral datasets.
Fig 1) Example of artificial generated from a pre-defined larva genotype
References
1. Masson, J.-B. et al. Identifying neural substrates of competitive interactions and sequence transitions during mechanosensory responses in Drosophila. PLOS Genet. 16, e1008589 (2020).
2. Jovanic, T. et al. Competitive Disinhibition Mediates Behavioral Choice and Sequences in Drosophila. Cell 167, 858-870.e19 (2016).
3. Croteau-Chonka, E. C. et al. High-throughput automated methods for classical and operant conditioning of Drosophila larvae. eLife 11, e70015 (2022).
4. Vogelstein, J. T. et al. Discovery of Brainwide Neural-Behavioral Maps via Multiscale Unsupervised Structure Learning. Science 344, 386–392 (2014).
5. Winding, M. et al. The connectome of an insect brain. Science 379, eadd9330 (2023).
6. Lehman, M. et al. Neural circuits underlying context-dependent competition between defensive actions in Drosophila larva. 2023.12.24.573276 Preprint at https://doi.org/10.1101/2023.12.24.573276 (2023).
7. Tredern, E. de et al. Feeding-state dependent modulation of reciprocally interconnected inhibitory neurons biases sensorimotor decisions in Drosophila. 2023.12.26.573306 Preprint at https://doi.org/10.1101/2023.12.26.573306 (2023).
8. Lake, B. M., Salakhutdinov, R. & Tenenbaum, J. B. Human-level concept learning through probabilistic program induction. Science 350, 1332–1338 (2015).
Scientific or technical background required for work program
The successful intern should have one of the following backgrounds:
- Statistical Physics, Applied Mathematics,
- Statistics & Bayesian Inference
Some fluency in Python is expected.
Contacts
dbc-epi-recrutement at pasteur dot fr
Similar Positions
-
Vortex Optiques : Défis Et Potentiels Pour Les Systèmes D'imagerie // Optical Vortices : Challenges And Potentials For Imaging Systems, Université de Bordeaux, France, 23 days ago
4 Apr 2024 Job Information Organisation/Company Université de Bordeaux Research Field Physics Researcher Profile Recognised Researcher (R2) Leading Researcher (R4) First Stage Researcher (R1) Esta...
-
Trois Essais Sur L'indépendance Des Banques Centrales // Three Essays On Central Bank Independance, Université de Bordeaux, France, 23 days ago
4 Apr 2024 Job Information Organisation/Company Université de Bordeaux Research Field Economics Researcher Profile Recognised Researcher (R2) Leading Researcher (R4) First Stage Researcher (R1) Es...
-
Determination Of The Specificity Of Small Molecule Splicing Modifiers Acting On A 1 Bulged 5’ Splice Sites And Rational Development Of Novel Splicing Modifiers., INSERM U1212-CNRS UMR 5320, France, about 1 month ago
27 Mar 2024 Job Information Organisation/Company INSERM U1212-CNRS UMR 5320 Research Field Chemistry » Biochemistry Biological sciences » Biological engineering Computer science » Informatics Rese...
-
High Intensity Ultrashort Electrical Pulses For New Biomedical Applications: Study And Optimization Of Electrical Parameters To Modulate Cellular Response, rosa orlacchio, France, 24 days ago
3 Apr 2024 Job Information Organisation/Company rosa orlacchio Research Field Engineering » Electronic engineering Biological sciences » Biological engineering Researcher Profile Recognised Resear...
-
Thesis In Polymer Chemistry (M/F): 'Biosourced Polyhydroxyurethanes As Encapsulation Materials For Photovoltaic Cells Bio Flex Pv', CNRS, France, 28 days ago
29 Mar 2024 Job Information Organisation/Company CNRS Department Laboratoire de chimie des polymères organiques Research Field Chemistry » Physical chemistry Physics » Chemical physics Physics » B...
-
M/F Fluorescent Nanoparticles As Biosensors, CNRS, France, 25 days ago
30 Mar 2024 Job Information Organisation/Company CNRS Department Institut des sciences moléculaires Research Field Chemistry » Physical chemistry Chemistry » Computational chemistry Researcher Pro...