Technician

This competition is open to all applicants, however internal candidates and applicants who were former employees of the University of Alberta in the past 18 months will be given priority consideration before external candidates. Please indicate your internal status using the "Advertisement" drop down menu when applying.

The Technician is primarily responsible for analyses of SARS-CoV-2 infected samples using quantitative cell imaging, genome-wide analyses, and protein-protein interactions. The successful applicant will be expected to undergo training to work in the Biosafety Level 3 laboratory (BSL3).

Duties

• Conduct infection and analyses of primary and continuous cell cultures in BSL3 facility;
• Maintain virus, cell culture stocks and cell culture equipment in BSL2 and BSL3 facilities;
• Perform molecular biological procedures for the construction and characterization of expression vectors;
• Participate in large-scale genetic and interactome screens using CRISPR and immunoaffinity purification/mass spectrometry;
• Analyses of large genetic and protein datasets;
• Perform experiments involving routine and slightly complex procedures in order to meet research objectives;
• Modify existing protocols for existing experiments in order to meet research objectives;
• Consult with principal investigator to develop new experiments and techniques for the research project;
• Use a variety of lab equipment to conduct experiments and monitor results;
• Collect and organize data on experiments and conduct preliminary analysis of large scale genetic and interactome screens;
• Prepare figures for manuscripts;
• Prepare and maintain a variety of specialized solutions for use in experiments;
• Perform other duties and responsibilities as requested by the principal investigator.

Qualifications and Experience

• Minimum BSc, MSc preferred.
• Minimum of two years lab experience.
• Experience with mammalian cell culture, molecular biology techniques required for protein expression in bacteria and animal cells.
• Experience with isolation of primary cell cultures from clinical samples.
• Experience in protein characterization methods, including polyacrylamide gel electrophoresis, immunoaffinity purification, and immunoblotting.
• Experience in advanced molecular biology techniques including cloning, quantitative polymerase chain reaction, next-generation sequencing.
• Experience in immunological techniques including Enzyme-Linked Immunosorbent Assays (ELISA) and flow cytometry.
• Experience in global proteome analyses and protein-protein interactions.
• Ability to analyze large data sets and pathway analyses using David, Gorilla, Partek Flow, Cytoscape, and/or Metascape.
• Experience in super-resolution and confocal immuno-fluorescence microscopy.
• Experience with image analyses software such as Volocity, Imaris and/or ImageJ.
• Experience with statistical analyses software such as GraphPad Prism and Excel.
• Experience in manuscript preparation and oral presentations using Microsoft Powerpoint, Adobe Photoshop or Illustrator, Biorender and/or other software for figure preparation.

Note: Online applications are accepted until midnight Mountain Standard Time of the closing date.

We thank all applicants for their interest; however, only those individuals selected for an interview will be contacted.