Investigations into atrial myopathy by Single Cell Sequencing.

Updated: 2 months ago
Deadline: 12 Mar 2021

(ref. BAP-2021-84)

Laatst aangepast : 2/02/2021

Organisational Unit: The Laboratory of Experimental Cardiology: The Laboratory of Experimental Cardiology is part of the cluster ‘Cardiac structure and function’ investigating cardiac structural, contractile and electrical remodeling processes during cardiovascular disease. This involves investigating from the cellular to the organ level, molecular mechanisms, left and right ventricular mechanics and impact of different interventions and anaesthetics. The laboratory is highly international consisting of post-doctoral scientists, PhD students and technicians. The laboratory is equipped with the wide range of expertise and skills required to manipulate and probe the transcriptome/epigenome (and the mechanisms governing functions and phenotype of the heart and cardiac myocyte fate choices). These resources span across mouse and large animal models of cardiac disease, GM mice, iPS cells, Single cell sequencing (10x and plate methods) RNA-Seq, ChiP-Seq, DNA (hydroxy)methylation analysis and analysis of excitation contraction coupling. You will receive extensive training and support in these areas. Through our excellent local and international collaborators and core facilities, you will have access to additional expertise and tools. The Department: The Department is situated in the Biomedical Campus of KU Leuven. It has the entire range of competences from basic science, over pre-clinical modeling to applied clinical and epidemiological research, taking a systems approach to the complexity of cardiovascular disorders. The University: KU Leuven offers a stimulating environment for international students and support for junior scientists to achieve their ambitions and in career development.


Project

Cell-Cell interactions in atrial myopathy and atrial fibrillation: We are recruiting a motivated graduate student to join on a new exciting collaborative multi-centre study to identify the cell-cell interactions that drive atrial remodelling during disease and ageing that leads to atrial fibrillation. The study will involve analysis of patient samples, preclinical models of AF as well as cell models. We will employ 10x sequencing approaches and in situ analysis to identify cell composition and cell state changes as well as changes in cells communication.Validation of findings will be in animal models and by mathematical modelling.Post-holders will receive training and gain experience in collaborative translational research and will be given opportunities to develop their skills at through interactions and visits/extended stays to partner laboratories. Suitable candidates that focus on wet or dry lab aspects of this project are invited to apply. Candidates with experience and/or knowledge in the areas of cardiovascular biology, next generation sequencing approaches including single cell analysis, bioinformatics and bioimaging are particularly encouraged to apply although enthusiasm and dedication is most sought after.

Responsibilities:

  • You will develop your own project in the area of atrial myopathy and transcriptomics with a particular aim to identify new regulatory nodes in the control of atrial remodelling and cell-cell signalling. 
  • You will collaborate with other members of the laboratory and with collaborators at other institutions. 
  • You will be expected to develop expertise in areas necessary for successful progress of your project. 
  • Depending on your area of research,you will be expected to perform experiments ranging from bioinformatic analysis, RNA sequencing to cell biology. 
  • You will be expected to present your work at conferences, lab meeting, meetings with collaborators and departmental meetings.

Profile

You are motivated, have an enquiring mind and are excited by science.
You have a masters degree in a relevant subject.
You have knowledge of NGS approaches, including RNA-Seq and/or bioinformatics.
You have knowledge/experience of NGS data processing and working knowledge of R and bioconductor packages would be an advantage. 
Experience in cardiovascular research including animal models is desirable.
You have a demonstrated ability to succeed.
You enjoy collaborating with your colleagues and externally to scientific advance.
You are a team player with excellent written and oral communication skills in English
You are an independent thinker who can drive a project forward.
You enjoy presenting your data to collaborators and to the community at scientific meetings.
You perform your research to the highest standards and integrity.


Offer

A fully funded 4 year PhD position.
A stimulating and supportive environment in which you acquire expertise as a multidisciplinary researcher.
Support to apply for independent funding.


Interested?

Please send your CV, motivation letter and 2 recommendation letters toProf. Dr. H. Llewelyn Roderick, Email: (Llewelyn.roderick@kuleuven.be). Please also applyvia the online portal of KU Leuven.


KU Leuven seeks to foster an environment where all talents can flourish, regardless of gender, age, cultural background, nationality or impairments. If you have any questions relating to accessibility or support, please contact us at diversiteit.HR@kuleuven.be.


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