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isolation/sequencing, cloning) across multiple organisms (E. coli, C. elegans, S. cerevisiae, and M. musculus). Lead protein expression, purification, and post-translational modification efforts. Identify and
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preparation, and standard E. coli genetic manipulations; carry out DNA and RNA extraction, quality assessment, and handling under RNase-free conditions; prepare RNA-seq and DNA sequencing libraries for both
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, chemistry, biology, etc.) Knowledge and experience in molecular biology The following skills would be an advantage: establishing bioassays, cloning, protein expression, working with cell cultures, E. coli and
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the evolution, cell biology, and virulence of bacterial pathogens, including Vibrio cholerae , enterohemorrhagic Escherichia coli , Shigella , Salmonella spp ., and Listeria monocytogenes . They take
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related lab-based activity. Background handling and maintaining stocks of model organisms, such as C. elegans, D. melanogaster, E. coli, or others. Familiar with molecular and cellular biological techniques
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proteins in E. coli and insect cells, and, where required, in mammalian cell culture. • Purification of proteins and protein complexes using a range of chromatographic methods (ÄKTA systems
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chemical materials and infectious diseases are present. Specific hazards of the position are, but are not limited to salmonella, Listeria, E. coli, Campylobacter, hot acids, bases, ethers and other chemicals
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multiple systems: bacterial (E. coli), mammalian (HEK293, HaCaT, A431), and insect cells (Sf9, baculovirus production). Purify challenging targets using ÄKTA SEC and affinity columns, perform sucrose
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with bacteriophages, E. coli and yeast. We seek a Staff Scientist to conduct experiments on molecular evolution under the supervision and guidance of the Principal Investigator, including assisting in
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works primarily with bacteriophages, E. coli and yeast. We seek a Staff Scientist to conduct experiments on molecular evolution under the supervision and guidance of the Principal Investigator, including