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experience in molecular biology methods, including E. coli culture, cloning, PCR, gel electrophoresis, and analysis of Sanger DNA sequencing results Targeted experience in basic immunological methods, such as
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topics to work on: (1) In vitro assays to determine inhibitor efficacy against redox membrane proteins (2) Cysteine enrichment of DsbA substrates in E. coli and Mycobacteria using proteomics. (3) determine
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laboratory work in recombinant protein expression in different platforms such as E. coli, yeast, protein analysis such as SDS-PAGE, Immunological assays such as ELISA, Western blot. Recombinant protein
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techniques. Perform laboratory work in recombinant protein expression in different platforms such as E. coli, yeast, protein analysis such as SDS-PAGE, Immunological assays such as ELISA, Western blot
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maintaining an evolution experiment with E. coli, requiring daily maintenance. Our lab studies evolutionary and ecological processes by conducting evolution experiments with bacteria. Duties include (i
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Molecular Biology at Penn State. The candidate in this position will study transcription and/or post-transcriptional regulatory mechanisms in B. subtilis and E. coli . Experience in gene regulation, in vitro
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and infectious diseases are present. Specific hazards of the position are, but are not limited to salmonella, Listeria, E. coli, Campylobacter, hot acids, bases, ethers and other chemicals. Special
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macrophages, neutrophils and T cells Cytokine biology and immune signaling pathways (e.g. G-protein signaling) Pathogens of interest include E. coli , S. pneumoniae , Legionella spp., and others. Projects will
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include expressing recombinant proteins in insect, mammalian, and E. coli cells; purifying proteins using FPLC systems; performing in vitro RNA transcription and labeling; carrying out in vitro assays
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preparation, and standard E. coli genetic manipulations; carry out DNA and RNA extraction, quality assessment, and handling under RNase-free conditions; prepare RNA-seq and DNA sequencing libraries for both