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experience of cell culture and common molecular biology techniques such as DNA/RNA isolation, ELISA, PCR, QPCR, Western blotting etc would be desirable although training will be given. No prior experience
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with immune assays and western blot; MS and AD human tissue bank brain and ChP samples for immunohistochemistry and fluorescence microscopy. Overall, the project explores exciting concepts in macrophage
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sequencing, qRT-PCR, western blotting, bioinformatics, ELISA, cell culture, statistics, and literature reviews. A working knowledge of these techniques is therefore desirable. We will compare subtypes, detect
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techniques such as immunohistochemistry and H-scoring, histopathological analysis, RNA sequencing, qRT-PCR, western blotting, bioinformatics, ELISA, cell culture, statistics, and literature reviews. A working
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analytical techniques, including molecular cloning, protein expression and purification, gel electrophoresis, western blotting, and HPLC. The research work will be performed in the Chemical and Synthetic
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use both invivo animal models and human adult tissue samples and will utilize a broad range of experimental methodologies including immunohistochemistry, fluorescence and electron microscopy and Western
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muscle), measurement of physical function through various laboratory-based tests, and molecular biology techniques (e.g. RT-qPCR and western blot analysis). There may also be the opportunity to apply
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, rotifers and/or tardigrades, microbiology, molecular biology and other lab skills (including, but not limited to, PCR, RT-qPCR, western blotting and microscopy), antimicrobial discovery and characterisation
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-time qPCR, and western blotting. The student will work within a well-funded and vibrant research environment in the David Greenfield Human Physiology Unit, the Nottingham Biomedical Research Centre