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techniques including cloning, PCR, qRT-PCR, Western blots and mammalian cell culture Experience with stem cell culture and differentiation protocols, particularly with iPSCs Experience with multiparametric
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cytometry, western blots, and quantitative PCR. Assist in the preparation of cell culture media and other buffer formulations. Perform analysis and documentation of research findings. Support the organization
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drug/gene delivery design and characterization. Evaluation of cellular response/interactions/functionality with scaffolds using western blotting, immunostaining, qPCR, fluorescent microscopy (confocal
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the successful completion of the research projects. The RA’s principal role will be to conduct research experiments which include but not limited staining, data acquisition, ELISA, cell culture, PCR, Western blot
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techniques (e.g., protein purification, Western blotting, immunofluorescent microscopy), willingness to learn new technologies. Competence: highly skilled in various basic wet laboratory techniques and capable
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• The project will be on effects of certain toxicants on cell cultured hepatic cells. The techniques involved include but not limited only to immunofluorescence, western blotting, RNA extraction and qPCR
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scientific publications in the field. Skills: Cell culture, molecular cloning, gene editing (CRISPR/Cas9), basic biochemical and cell biological techniques (e.g., protein purification, Western blotting
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, RT-PCR, western blotting etc.) Microscopy skills (ImageJ, quantitative Immunofluorescence microscopy) Knowledge of mechanobiology, senescence, DNA damage response pathways will be highly advantageous
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including western blotting, cloning, protein expression and purification, viral transfection, qRT-PCR, immunoprecipitation, immunofluorescence, immunohistochemistry, confocal microscopy imaging, etc
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and western blotting Any experience with analyses of human clinical samples, RNA sequencing, RNA immunoprecipitation, mass spectrometry and/or CRISPR genome editing is highly desirable A proven track