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POSITION OBJECTIVE Working under moderate supervision to perform biomedical research using skills in cell culture, molecular biology and protein chemistry and perform experiments on live animal
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cell culture (PCR, DNA analysis, protein analysis, retroviral/lentiviral construct and cloning/sub-cloning). (45%) Conduct analysis of samples. Generate, maintain and analyze tissue culture cell lines
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protein from cells, western blotting, and quantitative rtPCR. Organize samples and analyze data to contribute to large projects. Provide interpretation of data and suggest potential improvements
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vitro and in vivo. In vitro work will include standard tissue culture technique such as passaging cells and counting cells and harvesting RNA and protein from cells in culture for qPCR and western
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and Title: Jonathan Stamler, MD Professor of Medicine; Director, Institute for Transformative Molecular Medicine POSITION OBJECTIVE The NIH-funded project examines protein-S-nitrosylation in regulating
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of biomarker assays for neurodegenerative diseases. The Kraus lab studies the misfolded protein seeds of neurodegenerative diseases including Alzheimer’s, Parkinson’s disease and Lewy body dementias, chronic
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experiments. To closely work with the principal investigator and other laboratory personnel to ensure the smooth operation of the protein expression and chromatographic operations (HPLC and FPLC
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part of a larger project. Perform microbiological culture techniques, manipulation of DNA (PCR, cloning), protein gels and western blot analysis. May assist in developing improved techniques, project
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biochemistry and biophysics, bioengineering, mitochondrial biology, metabolism, and gene therapy. These positions are funded by the NIH and industry. Our laboratory investigates molecular and in vivo contractile
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vitro work will include standard tissue culture technique such as passaging cells and counting cells and harvesting RNA and protein from cells in culture for qPCR and western blotting. (65%) Record