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of Neuroscience at the University of Florida, Gainesville. The Scientist will be directly working with the Principal Investigator (Dr. Freddyson J. Martinez-Rivera) in establishing the foundations, team, techniques
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electrophoresis, and transformation; Protein Biochemistry including SDS-PAGE, Western Blotting and ELISA. Cell Culture and Cellular Biology Perform research experiments investigating mechanisms of action of channel
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diseases. Successful candidates will perform the vivo testing of drugs in animal models for diseases, perform molecular biological techniques to probe mitochondrial dynamics and function that include Western
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molecular biological experiments (i.e., Western Blot, PCR, qPCR, and FACS), coronary or pulmonary vascular cell isolation from mice, or cell physiological experiments (e.g., ROS, NOS, cytosolic Ca2
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or echocardiogram in mice, isolation of exosomes from human and animal blood), and molecular biological experiments (i.e., Western Blot, PCR, qPCR, or FACS). Additionally, the role involves troubleshooting technical
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, immunoprecipitation, immunofluorescence, Western blotting, FACS, mass spectrometry, transcriptomics, CRISPR-Cas9, etc. Perform data collection and analysis Initiate and maintain cell cultures Prepare and maintain
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of coronary and pulmonary endothelial cells from animals) and molecular biological experiments (i.e., Western Blot, PCR, qPCR, or FACS). Additionally, the role involves troubleshooting technical issues
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services to regional community under the University of Florida’s College of Medicine. Pensacola is a beautiful and vibrant coastal city in the western Florida panhandle. The family-friendly city has a
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are scheduled for review. Correspond with the IRB chairman as a representative of the PI when required. Liaison with the IRB, both the University of Florida (myIRB, IRB01) and the Western IRB to develop, edit
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hybridoma cell lines. Occasional after hours, weekend, and holiday post procedure monitoring required. - Develop and perform biochemical assays (e.g., ELISA and western blot). Analyze experimental data. Keep