27 Mar 2024
Job Information
- Organisation/Company
CNRS- Department
Institut des Neurosciences Cellulaires et Intégratives- Research Field
Neurosciences
Biological sciences- Researcher Profile
First Stage Researcher (R1)- Country
France- Application Deadline
16 Apr 2024 - 23:59 (UTC)- Type of Contract
Temporary- Job Status
Full-time- Hours Per Week
35- Offer Starting Date
1 Jul 2024- Is the job funded through the EU Research Framework Programme?
Not funded by an EU programme- Is the Job related to staff position within a Research Infrastructure?
No
Offer Description
The Cellular and Integrative Neuroscience Institute (INCI) is a CNRS research laboratory in partnership with the University of Strasbourg, comprising 9 teams (130 people), whose mission is to explore the nervous system and its pathologies. The candidate will be supervised by Philippe Isope and researchers from the Neuronal Network Physiology team.
The main aim of this thesis project is to study the functional consequences of dominant gain-of-function (GoF) mutations in the CACNA1G gene causing a severe neurodevelopmental disorder characterized by cerebellar atrophy in childhood (ChCA, also known as SCA42ND). CACNA1G encodes Cav3.1, a voltage-gated T-type calcium channel widely expressed in the nervous system, particularly in the cerebellum, cortex and thalamus. Cav3.1 is involved in electrical and calcium signaling in neurons, including synaptic depolarization, in connection with its dendritic localization.
We will study this mutation using a newly developed mouse model in which one of the mutations identified in humans has been introduced and is expressed under the control of CRE recombinase. In this way, we can express the mutation specifically in Purkinje cells of the cerebellum. Our preliminary data show that, under these conditions, the animals display disorders similar to those observed in humans. We will study how calcium signaling is affected in Purkinje cells and what the physiological consequences are. Experiments will be carried out in vitro on mouse cerebellum slices, or in vivo by combining electrophysiological recordings with calcium imaging using a biphoton microscope. In this way, we will be able to observe the consequences of this gain-of-function mutation on calcium signaling in Purkinje cell dendrites, as well as on the functioning of cerebellar networks in awake animals.
Requirements
- Research Field
- Neurosciences
- Education Level
- Master Degree or equivalent
- Research Field
- Biological sciences
- Education Level
- Master Degree or equivalent
- Languages
- FRENCH
- Level
- Basic
- Research Field
- Neurosciences
- Years of Research Experience
- None
- Research Field
- Biological sciences
- Years of Research Experience
- None
Additional Information
- Website for additional job details
https://emploi.cnrs.fr/Offres/Doctorant/UPR3212-PHIISO-010/Default.aspx
Work Location(s)
- Number of offers available
- 1
- Company/Institute
- Institut des Neurosciences Cellulaires et Intégratives
- Country
- France
- City
- STRASBOURG
- Geofield
Where to apply
- Website
https://emploi.cnrs.fr/Candidat/Offre/UPR3212-PHIISO-010/Candidater.aspx
Contact
- City
STRASBOURG- Website
http://inci.u-strasbg.fr/
STATUS: EXPIRED