PhD in Biology- Autophagy I2BC-Paris Saclay University M/F

23 May 2024
Job Information

Organisation/Company

CNRS

Department

Institut de Biologie Intégrative de la Cellule

Research Field

Physics

Researcher Profile

First Stage Researcher (R1)

Country

France

Application Deadline

12 Jun 2024 - 23:59 (UTC)

Type of Contract

Temporary

Job Status

Full-time

Hours Per Week

35

Offer Starting Date

1 Oct 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

Teame Autophagy and Development, Institute for Integfrative Biology of the Cell, CNRS, Avenue de la Terrasse 91198 Gif-sur –Yvette, France
https://www.i2bc.paris-saclay.fr/autophagy-and-development/

Title: Functional characterization of autophagosome populations by single event microscopy and proteomic approach

Summary: Autophagy is a major cellular mechanism of degradation of cytoplasmic constituents. It is an essential mechanism used by the cell to resist adverse conditions, nutritional, physical or chemical stress, by eliminating non-essential or damaged proteins and cellular organelles. Autophagy is also involved in many developmental processes and during aging, and its deregulation is linked to many pathologies (infections, cancer and neurodegenerative diseases). This process focusses on the formation of specialized vesicles called autophagosomes that sequester the cellular constituents to be recycled and then fuse with the lysosomal compartment for their degradation. Our team uses the nematode Caenorhabditis elegans, to characterize autophagic functions under physiological, stress or pathological conditions.
The production of autophagosomes is a complex, versatile and highly plastic dynamic process, mobilizing various membrane compartments. This PhD project aims to characterize in vivo and at the scale of single events the different autophagosome populations in order to dissect their specificities. It focuses on specific autophagic processes, characterized by our team, during development or for adaptation to thermal stress. Our studies of autophagosomal proteins of the LC3/GABARAP family of ubiquitin-like proteins showed the existence of at least three autophagosome populations whose functional specificities are unknown. The ambition of this project is to make an experimental leap from the global analysis of autophagy in the cell to that of the individual autophagosome.
This project is organized in two complementary axes in order to characterize the different types of autophagosomes. 1) Using CRISPR/Cas9 approaches, the laboratory has recently developed tools to analyze the dynamics of autophagosomes in vivo from their induction to their fusion with lysosomes. A 4D microscopy approach followed by a tracking image analysis method will allow to characterize single events during the autophagic flow and then to perform quantitative analyses.
2) In parallel, the use of specific LC3 and GABARAP traps, will allow to purify the different autophagosome populations and then to characterize their content by a proteomic approach.

1) Leboutet, R., Largeau, C., Müller, L., Prigent, M., Quinet, G., Rodriguez, M. S., Cuif, M. H., Hoppe, T., Culetto, E., Lefebvre, C., & Legouis, R. (2023). LGG-1/GABARAP lipidation is not required for autophagy and development in Caenorhabditis elegans. eLife, 12, e85748. https://doi.org/10.7554/eLife.85748
2) Chen, Y., Leboutet, R., Largeau, C., Zentout, S., Lefebvre, C., Delahodde, A., Culetto, E., & Legouis, R. (2021). Autophagy facilitates mitochondrial rebuilding after acute heat stress via a DRP-1-dependent process. The Journal of cell biology, 220(4), e201909139. https://doi-org/10.1083/jcb.201909139
3) Quinet, G., Génin, P., Ozturk, O., Belgareh-Touzé, N., Courtot, L., Legouis, R., Weil, R., Cohen, M. M., & Rodriguez, M. S. (2022). Exploring selective autophagy events in multiple biologic models using LC3-interacting regions (LIR)-based molecular traps. Scientific reports, 12(1), 7652. https://doi-org/10.1038/s41598-022-11417-z

Requirements

Research Field

Physics

Education Level

Master Degree or equivalent

Languages

FRENCH

Level

Basic

Research Field

Physics

Years of Research Experience

None

Additional Information

Website for additional job details

https://emploi.cnrs.fr/Offres/Doctorant/UMR9198-RENLEG-002/Default.aspx

Work Location(s)

Number of offers available

1

Company/Institute

Institut de Biologie Intégrative de la Cellule

Country

France

City

GIF SUR YVETTE

Geofield

Where to apply

Website

https://emploi.cnrs.fr/Candidat/Offre/UMR9198-RENLEG-002/Candidater.aspx

Contact

City

GIF SUR YVETTE

Website

http://www.i2bc.paris-saclay.fr

STATUS: EXPIRED