RESEARCH TECHNICIAN II

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Occ Summary

The Preston Robert Tisch Brain Tumor Center under the Department of Neurosurgery is seeking a full-time Research Technician II to join one of its labs. As a member of our highly collaborative team of researchers and clinicians, you will have the opportunity to work with cutting-edge technology and techniques to study the innate immune system, the impact of immunotherapy treatment and delivery of drugs in brain cancer. The lab focuses on biomarkers of immune response and novel treatments to increase patient survival.

Work Preformed

• Perform molecular assays including but not limited to nucleic acid extraction, PCR, and qPCR.
• Perform protein assays and western blotting.
• Perform cell culture on human and mouse cell lines.
• Perform work related to experiments on mouse models, including injections, surgery, monitoring behavior, and collection and preparation of tissues for histological analyses, RNA/DNA/protein isolation.
• Operate laboratory equipment required for specific tests.
• Prepare culture media, chemicals, reagents, and solutions.
• Document and compile data in electronic lab notebook from experimental results, utilizing ELN, Adobe Illustrator, BioRender, and MS Office software.
• Execute work independently after design input from supervisor or principal investigator.
• Review laboratory methods, scientific journals, abstracts, and other literature for information applicable to the implementation and design of research experiments.
• Be willing to learn and apply analytical skills to data utilizing GraphPad Prism
• Meet or communicate by e-mail with your physician-scientist supervisor regularly to plan experiments, troubleshoot problems, and discuss results.
• Participate in regular lab meetings to discuss results or articles in the literature.
• Perform other related duties as required by supervisor.

Other job responsibilities relevant to the lab include handling research specimens for ongoing clinical trials, hands-on experience visualizing genomic datasets, and mentoring undergraduate students in the lab.

Education, Skills & Experience

• Bachelor’s degree in biology, or related field.
• A background in cancer biology, cell culturing, molecular assays, and or mouse work a plus.
• Must adhere to strict safety requirements.
• Must be detail-oriented, well organized and perform work under Good Lab Practices.
• Should be comfortable working in teams and possess clear communication skills.
• Position could have weekend hours when working on mouse experiments.

Knowledge, Skills and Abilities

N/A

Minimum Qualifications
Education

Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.