Research Technician

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Job Summary

The Glover lab is seeking a Research Technician to work with Professor Glover to study the role of lysosome-related organelles in the delivery of microtubule organising molecules to centrioles to form functional centrosomes in Drosophila. 

Essential Job Duties

• Conduct moderately complex genetic crosses with Drosophila and apply behavioral assays and immunofluorescent microscopy on fixed tissue preparations from the progeny to determine resulting phenotypes.  Will review findings with lab management and discuss future direction for the next experiment.
• Use CRISPR/Cas9 mutagenesis to generate null and deletion mutants of genes for vesicle trafficking and centrosomal proteins.
• Under general supervision, identify domain structure of candidate regulatory proteins, discuss how to design mutations to test functions of regulatory regions with lab management, carry out in vitro mutagenesis to generate the corresponding mutant genes, and introduce such mutant genes into Drosophila.
• Perform advanced modifications and testing of in vitro protocols for vesicle trafficking and centrosomal maturation.

Basic Qualifications

• A bachelor’s degree majoring in biology, or a related subject is required.
• The successful applicant should have a minimum of 2 years of related experience.
• Core molecular techniques in DNA manipulation, immuno-histochemical staining, and confocal microscopy are essential.

Preferred Qualifications

• Experience working in a laboratory using Drosophila as a model system to study aspects of cell or developmental biology.
• Experience on working with lipid-associated proteins and in the use of Drosophila molecular genetics to analyze protein structure and function.
• Experience with behavioural assays (such as climbing and motility) would be advantageous. 

 Required Documents

• Resume