Life Science Research Professional 1

The  Rabinovitch lab in the Departments of Pediatrics  and the BASE Initiative is seeking a highly motivated Life Science Research Professional 1 to join my lab in collaboration with the Engreitz, Skylar-Scott and Nicolls laboratories in modeling disease using iPSC-derived vascular and inflammatory cells and in elucidating the molecular mechanisms that unerlie  the complex genetic and molecular mechanisms involved in vascular disease, particularly in response to genetic and environmental perturbations.  

The ultimate goal is to use this new knowledge to find therapies for this disease.  We use pluripotent stem cell and animal models to define the genes and regulatory elements necessary for cell fate decisions, and to probe how variation in these sequences can lead to pulmonary vascular disease. Experimental approaches utilized include various RNA-sequencing modalities (e.g. single-cell  RNA-seq), ChIP-sequencing, chromatin accessibility measurements (e.g. ATAC-sequencing) and high-throughput CRISPR screens. We work closely with physicians to ensure our experimental plans will solve critical problems faced by patients.

We are seeking a creative, driven, organized individual to aid in the design and implementation of these experiments. The LSRP1 will collaborate with the other team members to develop protocols, perform experiments, and evaluate and interpret results.  

The candidate for this position should have experience with standard molecular biology techniques and mammalian cell culture including work with stem cells and gene editing; excellent communication, organization, rigor and attention to detail and time management skills; and the ability to think creatively to solve problems.

Duties include:

·       Work collaboratively with team members to develop, design, and carry out experiments that are essential to the group’s collective success

·       Optimize and apply functional genomics technologies (e.g. RNA-seq, ChIP-seq, ATAC-seq, CRISPR)

·       Help with some mouse work

·       Interpret experimental results; document, summarize and present findings in individual and group meetings

·       Maintain a detailed organizational plan and record to ensure successful and timely completion of required tasks

·       Contribute to generation of protocols and publications particularly related to disease modeling using stem cell derived vascular and inflammatory cells

*- Other duties may also be assigned

All members of the Department of Pediatrics are engaged in continuous learning and improvement to foster a culture where diversity, equity, inclusion, and justice are central to all aspects of our work. The Department collectively and publicly commits to continuously promoting anti-racism and equity through its policies, programs, and practices at all levels.

Stanford University provides pay ranges representing its good faith estimate of what the University reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location, and external market pay for comparable jobs. The pay range for this position working in the California Bay area is $25.96 to $36.54.

•       MSc degree in biology or related field. Highly motivated candidates of all levels are encouraged to apply.

•       2+ years of experience in a research laboratory  with experience in stem cell differentiation  molecular biology techniques   mammalian cell culture and application of transfection and gene edigint with CRISPR  

•       Strong technical knowledge and experience with cell culture, cell and  molecular biology, and gnomic analyses, and ability to provide assistance with structural, functional and genomic analysis of mouse models of pulmonary vascular disease.

EDUCATION & EXPERIENCE (REQUIRED):

Bachelor's degree in related scientific field.

KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):

·       General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.

·       Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.

·       Ability to maintain detailed records of experiments and outcomes.

·       General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.

·       Ability to work under deadlines with general guidance.

·       Excellent organizational skills and demonstrated ability to accurately complete detailed work.

CERTIFICATIONS & LICENSES:

None

PHYSICAL REQUIREMENTS*:

·       Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.

·       Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.

·       Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.

·       Must have correctible vision to perform duties of the job.

·       Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.

·       Ability to lift heavy objects weighing up to 50 pounds.

·       Ability to work in a dusty, dirty, and odorous environment.

·       Position may require repetitive motion.

*- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.

WORKING CONDITIONS:

·       May require working in close proximity to blood borne pathogens.

·       May require work in an environment where animals are used for teaching and research.

·       Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.

·       Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.

·       May require extended or unusual work hours based on research requirements and business needs.