ASSISTANT PROFESSOR/ Post doc position

17 Apr 2024
Job Information

Organisation/Company

Warsaw University of Technology

Research Field

Biological sciences » Biological engineering
Biological sciences » Biology
Chemistry » Biochemistry
Technology » Biotechnology

Researcher Profile

Recognised Researcher (R2)

Country

Poland

Application Deadline

17 May 2024 - 16:00 (Europe/Warsaw)

Type of Contract

Temporary

Job Status

Full-time

Hours Per Week

40

Offer Starting Date

5 Jun 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

We are looking for a qualified postdoctoral researcher to join our dynamic research team to conduct advanced research in  metabolic engineering of S.cerevisiae (backer’s yeast)                           The person employed in this position will be responsible for the implementation of the research project and writing scientific publications.

The project will be focused on obtaining genetically modified strains of S.cerevisiae and characterising them. Genetic manipulations will require the candidate to design and prepare constructs for the CRISPR/Cas genome editing of S.cerevisiae. Genetic manipulations are aimed at obtaining a strain capable of simultaneous biosynthesis of mixed PHB/PLA (poly((R-3 hydroxybutyrate/polylactide) polymers. As part of the research conducted so far, the project leader's team has developed a strain with a favourable metabolic profile, which will serve as the starting point for genetic manipulation and for the production of the polymers. The effects of genome editing will be verified at the level of transcription using real-time PCR, at the level of metabolism by determining concentration of intracellular metabolites, and in the culture media, as well as at the level of activity of selected enzymes, in the synthetic pathways as well as their cellular levels using the western blot immunodetection method. For the most promising strains, quantitative tests of the synthesis of polylactide and hydroxybutyrate will be carried out, as well as the optimisation of polymer production efficiency using a laboratory bioreactor. The extraction and characterisation of polymers in terms of their composition will be carried out using chromatographic methods and MALDI-TOF mass spectrometry/ NMR. Optimising the polymer extraction process from S.cerevisiae will be a key step in the project, followed by an evaluation of the strategy in designing a synthetic hybrid microorganism as an efficient polymer producer. The project will be caried out over several interactions to obtain the optimal solution.

The postdoctoral researcher will be working in a friendly environment at the Chair of Drug and Cosmetics Biotechnology at the Faculty of Chemistry WUT.

Requirements

Research Field

Biological sciences » Biological engineering

Education Level

PhD or equivalent

Research Field

Biological sciences » Biology

Education Level

PhD or equivalent

Research Field

Chemistry » Biochemistry

Education Level

PhD or equivalent

Research Field

Technology » Biotechnology

Education Level

PhD or equivalent

Skills/Qualifications

1. PhD degree in a field related to the announced project (preferred disciplines are: biological sciences, life sciences, bioscience, biological engineering).

2. documented experience in conducting research in the area  which may be confirmed by scientific publications or references.

3. The candidate is expected to have an experience in at least two of the research directions listed below:

- microbial genome editing using the CRISPR/Cas technique

- molecular biology involving microorganisms (bacteria, yeast cells), working with DNA/RNA

- experience in using PCR/RT-PCR techniques

- experience of enzymatic assays

- analysis of PHB and PLA polymers

- extraction of polymers from microorganisms and fungi, and the chromatographic analysis of polymers

4. excellent communication skills, both written and oral, in English.

5. ability to cooperate effectively with other members of the research team and openness to sharing knowledge and experience.

6. commitment to conducting innovative research and willingness to develop in a given scientific field.

Additional advantage:

- Experience in writing scientific publication

Languages

ENGLISH

Level

Good

Languages

POLISH

Level

Good

Research Field

Biological sciences » Biological engineeringBiological sciences » BiologyChemistry » BiochemistryTechnology » Biotechnology

Years of Research Experience

4 - 10

Additional Information
Additional comments

More information is available from :

dr hab. inż. Malgorzata Adamczyk, prof. uczelni,  Gmach Technologii Chemicznej, ul. Koszykowa 75,

00-662 Warszawa, pok. 103A, tel.: (+48 22) 234-7677,

e-mail: [email protected]

Work Location(s)

Number of offers available

1

Company/Institute

Warsaw University of Technology, Faculty of Chemistry Chair of Drug and Cosmetics Biotechnology

Country

Poland

City

Warsaw

Postal Code

00 - 664

Street

Noakowskiego 3

Geofield

Where to apply

E-mail

[email protected]

Contact

City

Warsaw

Website

https://www.bip.pw.edu.pl/content/view/full/394

Street

Noakowskiego 3

Postal Code

00 - 664

E-Mail

[email protected]

STATUS: EXPIRED