Postdoctoral researcher in molecular virology : Dynamic of viral and cellular transcriptomes during HIV infection

14 Feb 2024
Job Information

Organisation/Company

INSERM DR IDF Centre Nord

Research Field

Biological sciences » Biology

Researcher Profile

First Stage Researcher (R1)

Country

France

Application Deadline

1 Apr 2024 - 18:00 (Europe/Paris)

Type of Contract

Temporary

Job Status

Full-time

Hours Per Week

38h30

Offer Starting Date

1 Jun 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

With over 38 million people infected worldwide, HIV remains a major public health issue. Antiviral therapies are effective in limiting virus replication, but its persistence in viral reservoirs hinders the hope of curing patients. The objective of the project developed by the postdoctoral researcher is to deepen knowledge of the molecular mechanisms which contribute to the regulation of the viral and cellular transcriptomes during viral replication and latency. The research program will be based on techniques in cell biology (cell culture, isolation of T lymphocytes from blood samples), virology (viral production and infections), molecular biology (RT-qPCR, Western blot, immunopurification of complexes...), flow cytometry, imaging (immunofluorescence), as well as sequencing analyses (short and long reads).

The following activities are available :

• Conduct a research project to understand the dynamic of HIV and cellular transcriptomes during infection: Formulate hypotheses related to the research project within the context of existing literature, design the experimental approach, conduct experiments, process data, and analyze the results.
• Provide regular reports on experimental results to the project supervisor and during laboratory meetings
• Disseminate and showcase the results (reports, contribution to figure formatting, scientific article writing, participation in conferences).
• Supervision and training of students/Ph.D. candidates.

Job specification and environment : 

• High throuput sequencing analyses
• Work in BSL2 and BSL3 environment
• Work with blood samples
• Possible variability in working hours

Requirements

Research Field

Biological sciences » Biology

Education Level

PhD or equivalent

Skills/Qualifications

Knowledge : 

• Good understanding of molecular biology, genomic, transcriptomic and preferably specializing in virology/HIV.
• Knowledge and expertise in bioinformatic would be a strong asset.
• Proficiency in spoken and written English

Skills :

• Skills in experimental laboratory work with the ability to independently plan and execute experiments.
• Previous experience in working in BSL2 environment is required and experience in BSL3 is preferred or a willingness to be trained for work in BSL3 conditions.
• Previous experience in molecular biology, cellular biology, and biochemistry (cloning, western blot, RT-qPCR, isolation and analysis of protein and ribonucleoprotein complexes, gene silencing or editing, siRNA/shRNA/CRISPR-CAS9) is required.
• Computer skills and proficiency in biology software, with a willingness to learn new programs and software as necessary.
• Proficiency in flow cytometry technology and immunofluorescence would be a plus.

Ability : 

• Organizational skills, ability to prioritize multiple tasks and set and meet deadlines.
• Ability to analyze information, write clear and concise research reports, documents and prepare research papers.
• Communications skills and ability to work well collaboratively with colleagues from multidisciplinary background

Experience required :

• PhD in molecular virology
• Bioinformatic skills 

Diploma level and training : 

• PhD
• Virology, molecular biology, transcriptomic, bioinformatic 

Languages

ENGLISH

Level

Excellent

Additional Information
Additional comments

The job is available between June et September.

Contract workers: from 2997,20 € gross per month depending on professional experience in equivalent positions (to be completed with your HR manager).

The opening position is funded by the ANRS.

/ Applicants should send their reseume, and cover letter and name of 2 references:

• Sarah Gallois-Montbrun

• Email : [email protected]  

Website for additional job details

https://institutcochin.fr/

Work Location(s)

Number of offers available

1

Company/Institute

Institut Cochin

Country

France

State/Province

Ile-de-France

City

Paris

Postal Code

75014

Street

22 rue Mechain

Geofield

Where to apply

E-mail

[email protected]

Contact

State/Province

Ile-de-France

City

Paris

Website

http://www.idf.inserm.fr/rubriques/l-inserm-en-ile-de-france/delegation-paris-5

Street

88 rue Regnault

Postal Code

75013

E-Mail

[email protected]

STATUS: EXPIRED