Fixed-term contract Post-Doctoral student in cellular microbiology (M/F)

27 Mar 2024
Job Information

Organisation/Company

CNRS

Department

Institut de Biologie Intégrative de la Cellule

Research Field

Chemistry » Biochemistry
Physics » Biophysics
Biological sciences » Biological engineering

Researcher Profile

Recognised Researcher (R2)

Country

France

Application Deadline

16 Apr 2024 - 23:59 (UTC)

Type of Contract

Temporary

Job Status

Full-time

Hours Per Week

35

Offer Starting Date

1 May 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

The post-doc will lead a project aimed at characterizing the interactions between effector proteins of the Shigella bacteria and the proteins of the host cell associated with the phagocytosis vacuole.
The recruited person will work on in vitro models of epithelial cell cultures infected by bacteria, bacterial extracts or purified compounds. The complexes stabilized by chemical bridging will be analyzed by mass spectrometry.
The post-doc will analyze the effects linked to these interactions on the intracellular replication of bacteria by fluorescence microscopy.

The post doc will have the following activities:
• Study pathogenic bacteria-host cell interactions in cell culture models.
• Conduct, by adapting the experimental conditions, a set of techniques (electrophoresis, immunological, histological techniques, cloning, purification of recombinant proteins, cell culture, manipulation of class 2 pathogenic bacteria (Shigella), fluorescence microscopy)
• Exploit and present analysis results
• Writing experience or study reports
• Generate and organize technical resources as part of the project
• Supervise internally and externally the principles and implementation of techniques associated with the project
• Ensure monitoring and transmission of results.
• Ensure scientific and technological monitoring in the field associated with the project
• Participate in the dissemination and promotion of results in the form of oral presentations and publications

The main research theme of the I2BC laboratory concerns the characterization of the integrated functioning of the cell and in particular the understanding of the processes which, at the molecular level, govern the organization and overall physiology of the cell.
Researchers carry out their work on different organisms (from viruses to human cells) or on complexes reconstituted in vitro, at different scales and under various physiological conditions.
The Department of Cellular Biology within the I2BC includes eight teams which study the molecular actors and mechanisms which govern biogenesis, the organization and maintenance of specialized structures and compartments of the cell, intracellular trafficking and exchanges between compartments, the actin cytoskeleton in physiological and pathological conditions.
The CaSMI (Calcium signaling and Microbial Infections) team associated with the Department of Cellular Biology studies the interactions between pathogenic bacteria and host cell leading to the crossing of the intestinal barrier in the case of the enteroinvasive bacteria Shigella and the blood-brain barrier by the pneumococcus .

Requirements

Research Field

Chemistry

Education Level

PhD or equivalent

Research Field

Physics

Education Level

PhD or equivalent

Research Field

Biological sciences

Education Level

PhD or equivalent

Languages

FRENCH

Level

Basic

Research Field

Chemistry » Biochemistry

Years of Research Experience

1 - 4

Research Field

Physics » Biophysics

Years of Research Experience

1 - 4

Research Field

Biological sciences » Biological engineering

Years of Research Experience

1 - 4

Additional Information
Eligibility criteria

Knowledge
• Microbiology, Biochemistry, Cell Biology
• Health and safety regulations
• Legal and ethical framework
• English language: B1 to B2 (common European framework of reference for languages)

Operational skills
• Protein purification techniques (ion exchange affinity chromatography)
• Cell biology techniques (cell culture, transfection)
• Knowledge of the research environment and scientific English
• Ability to interact with students and researchers

Expertise
• Cell culture techniques
• Immunofluorescence markings
• Mastery of protein manipulation techniques
• Skills

Know-how
• Show rigor
• Have a sense of organization
• Know how to work in a team
• Know how to adapt

Additional comments

-

Website for additional job details

https://emploi.cnrs.fr/Offres/CDD/UMR9198-GUYTRA-003/Default.aspx

Work Location(s)

Number of offers available

1

Company/Institute

Institut de Biologie Intégrative de la Cellule

Country

France

City

GIF SUR YVETTE

Geofield

Where to apply

Website

https://emploi.cnrs.fr/Candidat/Offre/UMR9198-GUYTRA-003/Candidater.aspx

Contact

City

GIF SUR YVETTE

Website

http://www.i2bc.paris-saclay.fr

STATUS: EXPIRED