Post-Doctoral Fellowship in Biochemistry and Molecular Biology of Trypanosomatids

19 Mar 2024
Job Information

Organisation/Company

FAPESP - São Paulo Research Foundation

Research Field

Chemistry

Researcher Profile

Established Researcher (R3)

Country

Brazil

Application Deadline

8 Apr 2024 - 23:59 (UTC)

Type of Contract

To be defined

Job Status

Not Applicable

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

The conversion of Cysteine to Pyruvate can be carried out by two enzymes, a broad-spectrum substrate Aspartate aminotransferase (ASAT), which includes Cys among them, and a Mercaptopyruvate Sulfotransferase (MPST). Due to their characteristics, ASAT of T. cruzi are potential candidates to catalyze the Cystein deamination. However, despite a putative sequence for these enzymes in the T. cruzi genome, there is currently no literature data showing this pathway. The goal of this project is to confirm this “direct” pathway for Pyruvate production from Cystein, and the possible existence of a dual connection between Serine and Cystein: through the enzyme cystathionine-β-synthase, Serine could be converted to Cystein (and subsequently to Pyruvate) or conversely, through the enzyme Cystein-γ-lyase (CGL) and the reverse action of cystathionine-β-synthase (CBS), Cystein could provide Serine, which could also provide Pyruvate through a Serine dehydrogenase. The reason for the co-existence of both pathways is not clear, as it seems that both primarily function to provide Pyruvate, one using Ser as intermediates and the other using Cystein. The goal of this project is to explore these pathweays using methods in enzymology, biochemistry, metabolomics, bioenergetics and gene edition by CRISPR/Cas9.

This opportunity is open to candidates of any nationality. The selected candidate will work at the University of São Paulo's Biomedical Sciences Institute (ICB-USP) in Brazil and receive a Post-Doctoral fellowship from the São Paulo Research Foundation (FAPESP) in the amount of R$ 9,047.40 monthly and a research contingency fund, equivalent to 10% of the annual value of the fellowship which should be spent on items directly related to the research activity. 
 
More information about the fellowship is at: fapesp.br/oportunidades/6861 .

Requirements
Additional Information
Eligibility criteria

Eligible destination country/ies for fellows:

• Brazil

Eligibility of fellows: country/ies of residence:

• All

Eligibility of fellows: nationality/ies:

• All

Work Location(s)

Number of offers available

1

Company/Institute

Instituto de Ciências Biomédicas, Universidade de São Paulo (ICB-USP)

Country

Brazil

State/Province

São Paulo

City

São Paulo

Postal Code

05508-000

Street

Av. Prof. Lineu Prestes, 1374

Geofield

Where to apply

Website

http://www.fapesp.br/oportunidades/6861

Contact

State/Province

São Paulo

City

São Paulo

Website

http://www.fapesp.br/oportunidades/

Street

R. Pio XI, 1500 - Alto da Lapa

Postal Code

05468-901

STATUS: EXPIRED