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in efficacy studies downstream processing (quantitative PCR, western blotting, histology) of preserved tissues, as well as analysis of signaling pathways (by western blotting, immune-fluorescence
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/or SDS-polyacrylamide gel electrophoresis and blotting; mammalian and/or bacterial cell culture and analysis; fluorescence and light microscopy; experience with personal computer hardware and software
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blotting; mammalian and/or bacterial cell culture and analysis; fluorescence and light microscopy; experience with personal computer hardware and software for word processing, data storage and analysis
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such as PCR, site-directed mutagenesis, DNA preparation Sanger sequencing of plasmid DNA Biotinylation and fluorescent labeling of recombinant proteins Flow cytometry analysis of fluorescent protein
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, primary cells, freezing cells, transfection, clonal isolation), histology, immunohistochemistry/immunofluorescence, flow cytometry, microscopy (fluorescence, confocal), mouse work (general care, breeding
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Caenorhabditis elegans. The intern will work with a mentor to exposure the worms to the metals, and then use transgenic strains of worms with fluorescent protein permitting visualization of neuronal structure
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mammalian cells. We are currently developing vaccines against HIV, influenza, and emergent coronaviruses. To study B cell signaling in response to antigens, we utilize molecular tools (fluorescent biosensors
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working knowledge in cellular immunological and molecular biology techniques including flow cytometry, fluorescence/confocal microscopy, PCR and vector cloning for receptor expression. Experience in cell